抄録
Offer Organization: Japan Society for the Promotion of Science, System Name: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Category: Grant-in-Aid for Scientific Research (A), Fund Type: -, Overall Grant Amount: - (direct: 33300000, indirect: 9990000)
We already developed super artificial restriction enzyme. In this research, we added affinity separating ability to this tool, and constructed the second- generation super artificial restriction enzyme. By modifying biotin to PNA which is a constituent of super restriction enzyme, we successfully extracted desired DNA fragments of several thousand base pairs in high purity and high yield from the human genome with 3 billion base pairs. Furthermore, by combining this biotin-modified PNA with S1 nuclease, we also succeeded in extracting desired DNA fragments from the human genome in one pot.