抄録
Offer Organization: Japan Society for the Promotion of Science, System Name: Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Category: Grant-in-Aid for Challenging Exploratory Research, Fund Type: -, Overall Grant Amount: - (direct: 2900000, indirect: 870000)
Protein-based molecular tools were developed, aiming to establish the IP3 clamp method, a novel technique to control the intracellular IP3 concentrations in living cells by light. It was shown that the combination of human phospholipase C and plant photoreceptor proteins could give the photoactivatability to an IP3-producing enzyme. We also developed fluorescent IP3 probes that have favorable excitation/emission wavelengths to be used with photoactivatable enzymes in the IP3 clamp system.