抄録
Offer Organization: Japan Society for the Promotion of Science, System Name: Grants-in-Aid for Scientific Research, Category: Grant-in-Aid for Young Scientists (B), Fund Type: competitive_research_funding, Overall Grant Amount: - (direct: 3200000, indirect: 960000)
We examined whether lactate causes skeletal muscle mitochondrial biogenesis, using extracted mouse muscle incubation system and electric stimulation system to C2C12 myotubes. As a result, 24h-lactate stimulation (10 mM) for extracted mouse muscle suppressed mRNA contents of PGC-1alpha and mitochondria. However lactate concentration exceeded 25 mM after 24 hours of lactate incubation, which was non-physiological high lactate concentration. Elevation in lactate concentration due to electrical stimulation increased the mRNA contents of PGC-1alpha in C2C12 myotubes. These results suggest that the increase in lactate concentration associated with muscle contraction may cause mitochondrial biogenesis via transcriptional regulation. It is necessary to clarify the dose response of lactate concentration in further studies.